A Key Role for NMN in Alleviating Organelle Miscommunication in HFD Mouse Liver
01 Jan

A Key Role for NMN in Alleviating Organelle Miscommunication in HFD Mouse Liver

Introduction

Recent research unveils that hepatic insulin resistance (IR) and steatosis in obesity have a strong association with endoplasmic reticulum (ER)-mitochondria miscommunication. Notably, β-nicotinamide mononucleotide (NMN) can ameliorate hepatic IR and steatosis to regulate obesity via reinforcing the interaction between ER and mitochondria.



About ER-mitochondria miscommunication

ER and mitochondria interact at contact sites called mitochondria-associated membranes (MAMs), where they exchange phospholipids and calcium (Ca2+). MAM acts as a bridge between the ER and mitochondria, participating in the regulation of calcium signaling, lipid homeostasis, mitochondrial dynamics, mitochondrial autophagy, and ER stress response. Disrupting ER-mitochondria communication may induce hepatic IR and steatosis.

IFrame  

Research protocol

In vivo, thirty 8-week-old male C57BL/6 J specific pathogen-free mice are fed with normal/high-fat diet (HFD) and normal/NMN-containing drinking water for 30 weeks.  During the experiments, the body weight, diet, food intake, appearance, and behavior characteristics of the experimental mice are recorded weekly. A week prior to the end of study, 12-h-fasting mice are subjected with intraperitoneal injection of glucose (2 mg/g body weight) or insulin (0.75 mU/g body weight) for glucose tolerance test and insulin tolerance test, followed by the measurement of glycemia using a glucometer.

In vitro, mouse normal hepatocytes NCTC1469 are seeded in 96-well microtiter plates for 12-h culture, then blended with specific palmitic acid (PA) for 36 h to establish the HFD model, and treated with NMN (500, 50, 5, 0.5, and 0.05 μM) for 24 h, followed by detection of physiological indicators.

Regulatory impacts of NMN upon obesity

In vivo, NMN ameliorates hyperlipidemia, hepatic steatosis, liver physiological metabolic damage, liver lipid metabolic abnormalities, and ER-mitochondrial miscommunication in the liver of HFD mice.
In vitro, NMN reduces PA-induced lipid accumulation, yet promotes PA-induced mitochondrial-ER communication in NCTC1469 cells.

 

 

NMN alleviates organelle miscommunication by increasing MAMs in HFD mice liver. Concretely, NMN ameliorates mitochondrial dysfunction and ER oxidative stress in the liver of HFD mice by increasing hepatic nicotinamide adenine dinucleotide (NAD+) level. This effect increases the MAMs between ER and mitochondria, thereby promoting intracellular ATP production and mitigating lipid metabolic disturbances in the liver of HFD mice.

Conclusion

NMN enhances the dynamic properties of MAMs to increase the communication between mitochondria and ER, ultimately reversing HFD-induced glycolipid metabolic disorder, IR and hepatic steatosis. 

Reference

[1] Beaulant A, Dia M, Pillot B, et al. Endoplasmic reticulum-mitochondria miscommunication is an early and causal trigger of hepatic insulin resistance and steatosis. J Hepatol. 2022;77(3):710-722. DOI:10.1016/j.jhep.2022.03.017
[2] Lam BCC, Lim AYL, Chan SL, et al. The impact of obesity: a narrative review. Singapore Med J. 2023;64(3):163-171. DOI: 10.4103/singaporemedj.SMJ-2022-232
[3] Li Y, Tian X, Yu Q, et al. Alleviation of hepatic insulin resistance and steatosis with NMN via improving endoplasmic reticulum-mitochondria miscommunication in the liver of HFD mice. Biomed Pharmacother. Published online May 3, 2024. DOI:10.1016/j.biopha.2024.116682

BONTAC NMN

BONTAC is the pioneer of NMN industry and the first manufacturer to launch NMN mass production, with the first whole-enzyme catalysis technology around the world. BONTAC NMN is patent-grade and has obtained the self-affirmed GRAS certification of FDA. At present, BONTAC has become the leading enterprise in niche areas of coenzyme. Our services and products have been highly recognized by global partners. Furthermore, BONTAC has the first national and the only provincial independent coenzyme engineering technology research center in Guangdong, China. The coenzyme products of BONTAC are widely used in fields such as nutritional health, biomedicine, medical beauty, daily chemicals and green agriculture.

Disclaimer

This article is based on the reference in the academic journal. The relevant information is provided for sharing and learning purposes only, and does not represent any medical advice purposes. If there is any infringement, please contact the author for deletion. The views expressed in this article do not represent the position of BONTAC. Under no circumstances will BONTAC be responsible for any claims, damages, losses, expenses or costs resulting directly or indirectly from your reliance on the information and material on this website.

Tags: nmn powder nad powder nmn manufacturer nad manufacturer nmn supplement

best nad powder coenzyme best nad powder factory best nad powder manufacturer best nad powder supplement best nad powder supplier buy nad powder coenzyme buy nad powder factory buy nad powder manufacturer buy nad powder supplement buy nad powder supplier high purity nad powder coenzyme high purity nad powder factory high purity nad powder manufacturer high purity nad powder supplement high purity nad powder supplier high quality nad powder coenzyme high quality nad powder factory high quality nad powder manufacturer high quality nad powder supplement high quality nad powder supplier purchase nad powder coenzyme purchase nad powder factory purchase nad powder manufacturer purchase nad powder supplement purchase nad powder supplier
best nadh powder coenzyme best nadh powder factory best nadh powder manufacturer best nadh powder supplement best nadh powder supplier buy nadh powder coenzyme buy nadh powder factory buy nadh powder manufacturer buy nadh powder supplement buy nadh powder supplier high purity nadh powder coenzyme high purity nadh powder factory high purity nadh powder manufacturer high purity nadh powder supplement high purity nadh powder supplier high quality nadh powder coenzyme high quality nadh powder factory high quality nadh powder manufacturer high quality nadh powder supplement high quality nadh powder supplier purchase nadh powder coenzyme purchase nadh powder factory purchase nadh powder manufacturer purchase nadh powder supplement purchase nadh powder supplier
best nmnh powder coenzyme best nmnh powder factory best nmnh powder manufacturer best nmnh powder supplement best nmnh powder supplier buy nmnh powder buy nmnh powder coenzyme buy nmnh powder factory buy nmnh powder manufacturer buy nmnh powder supplement buy nmnh powder supplier high purity nmnh powder coenzyme high purity nmnh powder factory high purity nmnh powder manufacturer high purity nmnh powder supplement high purity nmnh powder supplier high quality nmnh powder high quality nmnh powder coenzyme high quality nmnh powder factory high quality nmnh powder manufacturer high quality nmnh powder supplement high quality nmnh powder supplier hot sale nmnh powder longevity nmnh powder coenzyme longevity nmnh powder factory longevity nmnh powder manufacturer longevity nmnh powder supplement longevity nmnh powder supplier nmnh powder company nmnh powder manufacturer
purchase nmn manufacturer supplier high quality nmn manufacturer supplement purchase nmn manufacturer factory purchase nmn manufacturer coenzyme longevity nmn manufacturer supplier longevity nmn manufacturer production high quality nmn manufacturer factory purchase nmn manufacturer production high purity nmn manufacturer supplement purchase nmn manufacturer supplement high purity nmn manufacturer factory high purity nmn manufacturer production best nmn manufacturer coenzyme best nmn manufacturer supplement best nmn manufacturer supplier buy nmn manufacturer coenzyme buy nmn manufacturer supplement buy nmn manufacturer production best nmn manufacturer production high purity nmn manufacturer coenzyme high quality nmn manufacturer supplier high purity nmn manufacturer supplier longevity nmn manufacturer factory longevity nmn manufacturer coenzyme longevity nmn manufacturer supplement high quality nmn manufacturer production high quality nmn manufacturer coenzyme buy nmn manufacturer factory buy nmn manufacturer supplier best nmn manufacturer factory
best nad manufacturer best nad manufacturer coenzyme best nad manufacturer factory best nad manufacturer supplement best nad manufacturer supplier buy nad manufacturer coenzyme buy nad manufacturer factory buy nad manufacturer production buy nad manufacturer supplement buy nad manufacturer supplier high purity nad manufacturer coenzyme high purity nad manufacturer factory high purity nad manufacturer production high purity nad manufacturer supplement high purity nad manufacturer supplier high quality nad manufacturer coenzyme high quality nad manufacturer factory high quality nad manufacturer production high quality nad manufacturer supplement high quality nad manufacturer supplier best nad manufacturer production
best nadh manufacturer best nadh manufacturer coenzyme best nadh manufacturer factory best nadh manufacturer supplement best nadh manufacturer supplier buy nadh manufacturer coenzyme buy nadh manufacturer factory buy nadh manufacturer production buy nadh manufacturer supplement buy nadh manufacturer supplier high purity nadh manufacturer coenzyme high purity nadh manufacturer factory high purity nadh manufacturer production high purity nadh manufacturer supplement high purity nadh manufacturer supplier high quality nadh manufacturer coenzyme high quality nadh manufacturer factory high quality nadh manufacturer production high quality nadh manufacturer supplement high quality nadh manufacturer supplier purchase nadh manufacturer coenzyme purchase nadh manufacturer factory purchase nadh manufacturer production purchase nadh manufacturer supplement purchase nadh manufacturer supplier best nadh manufacturer production
high quality nmnh manufacturer coenzyme longevity nmnh manufacturer coenzyme purchase nmnh manufacturer supplier longevity nmnh manufacturer supplement high quality nmnh manufacturer production longevity nmnh manufacturer factory high purity nmnh manufacturer factory high purity nmnh manufacturer coenzyme high quality nmnh manufacturer supplement high purity nmnh manufacturer supplement purchase nmnh manufacturer factory high purity nmnh manufacturer production best nmnh manufacturer factory best nmnh manufacturer coenzyme longevity nmnh manufacturer production high quality nmnh manufacturer factory buy nmnh manufacturer supplier purchase nmnh manufacturer production purchase nmnh manufacturer coenzyme buy nmnh manufacturer supplement best nmnh manufacturer production high purity nmnh manufacturer supplier high quality nmnh manufacturer supplier buy nmnh manufacturer factory buy nmnh manufacturer coenzyme best nmnh manufacturer supplement best nmnh manufacturer supplier longevity nmnh manufacturer supplier purchase nmnh manufacturer supplement buy nmnh manufacturer production
what is nmn supplement top selling nmn supplement top quality nmn supplement nmn supplement supplier nmn supplement production nmn supplement price nmn supplement manufacturer nmn supplement in china nmn supplement for sale nmn supplement factory nmn supplement david sinclair nmn supplement company hot sale nmn supplement high quality nmn supplement china nmn supplement buy nmn supplement best price nmn supplement best nmn supplement david sinclair best nmn supplement