NMNH: 1. “Bonzyme” Whole-enzymatic method, environmental-friendly, no harmful solvent residues manufacturing powder. 2. Bontac is a very first manufacture in the world to produce the NMNH powder on the level of high purity, stability. 3. Exclusive “Bonpure” seven-step purification technology, high purity(up to 99%) and stability of production of NMNH powder 4. Self-owned factories and obtained a number of international certifications to ensure high quality and stable supply of products of NMNH powder 5. Provide one-stop product solution customization service
NADH: 1. Bonzyme whole-enzymatic method, environmental-friendly, no harmful solvent residues 2. Exclusive Bonpure seven-step purification technology, purity up higher than 98 % 3. Special patented process crystal form, higher stability 4. Obtained a number of international certifications to ensure high quality 5. 8 domestic and foreign NADH patents, leading the industry 6. Provide one-stop product solution customization service
NAD: 1. “Bonzyme” Whole-enzymatic method, environmental-friendly, no harmful solvent residues 2. Stable supplier of 1000+ enterprises around the world 3. Unique “Bonpure” seven-step purification technology, higher product content and higher conversion rate 4. Freeze drying technology to ensure stable product quality 5. Unique crystal technology, higher product solubility 6. Self-owned factories and obtained a number of international certifications to ensure high quality and stable supply of products
NMN: 1. “Bonzyme”Whole-enzymatic method, environmental-friendly, no harmful solvent residues 2. Exclusive“Bonpure”seven-step purification technology, high purity(up to 99.9%) and stability 3. Industrial leading technology: 15 domestic and international NMN patents 4. Self-owned factories and obtained a number of international certifications to ensure high quality and stable supply of products 5. Multiple in vivo studies show that Bontac NMN is safe and effective 6. Provide one-stop product solution customization service 7. NMN raw material supplier of famous David Sinclair team of Harvard University
Bontac Bio-Engineering (Shenzhen) Co., Ltd. (hereafter referred to as BONTAC) is a high-tech enterprise established in July 2012. BONTAC integrates R&D, production and sales, with enzyme catalysis technology as the core and coenzyme and natural products as main products. There are six major series of products in BONTAC, involving coenzymes, natural products, sugar substitutes, cosmetics, dietary supplements and medical intermediates.
As the leader of the global NMN industry, BONTAC has the first whole-enzyme catalysis technology in China. Our coenzyme products are widely used in health industry, medical & beauty, green agriculture, biomedicine and other fields. BONTAC adheres to independent innovation, with more than 170 invention patents. Different from the traditional chemical synthesis and fermentation industry, BONTAC has advantages of green low-carbon and high-value-added biosynthesis technology. What’s more, BONTAC has established the first coenzyme engineering technology research center at the provincial level in China which also is the sole in Guangdong Province.
In the future, BONTAC will focus on its advantages of green, low-carbon and high-value-added biosynthesis technology, and build ecological relationship with academia as well as upstream/downstream partners, continuously leading the synthetic biological industry and creating a better life for human beings.
1、As an advanced technology NAD manufacturer, BONTAC choose the Enzymatic method, environmental-friendly, no harmful solvent residues manufacturing powder
2、High purity(up to 99%) and stability of production of NAD powder
3、Self-owned factories and obtained a number of international certifications to ensure high quality and stable supply of products of NAD powder
4、Multiple in vivo studies show that Bontac NAD powder is safe and effective
5、Provide one-stop product solution customization service
Molecules that can be taken in supplement form to increase NAD levels in the body are referred to by some as “NAD boosters.” Studies conducted over the past six decades suggest that the following are some of the many benefits associated with taking an NAD supplement:
Can Help Restore Mitochondrial Function
Helps Repair Blood Vessels —A 2018 mice study found that supplementation could aid in repair and growth of aged blood vessels. There’s also some evidence it can help manage heart disease risk factors like high blood pressure and high cholesterol.
May Improve Muscle Function — One animal study conducted in 2016 found that degenerative muscles had improved muscle function when supplemented with NAD+ precursors.
Potentially Helps Repair Cells and Damaged DNA — Some studies have found evidence that NAD+ precursor supplementation leads to an increase in DNA damage repair. NAD+ is broken down into two component parts, nicotinamide and ADP-ribose, which combine with proteins to repair cells.
May Help Improve Cognitive Function — Several studies conducted on mice have found that mice treated with NAD+ precursors experienced improvements in cognitive function, learning and memory. Findings have led researchers to believe that NAD supplement may help protect against cognitive decline/Alzheimer’s disease.
May Help Prevent Age-Related Weight Gain — A 2012 study showed that when mice fed a high-fat diet were given an NAD supplement, they gained 60 percent less weight than they did on the same diets without the supplement. One reason this may be true is that nicotinamide adenine dinucleotide helps regulate production of stress- and appetite-related hormones, thanks to its effects on circadian rhythms.
Precursors are molecules used in chemical reactions inside the body to create other compounds. There are a number of precursors of NAD+ that result in higher levels when you consume enough of them.
The preparation methods utilized by manufacturers worldwide of NAD powder are mainly divided into chemical synthesis method and biocatalytic method, among which biocatalytic method includes biological fermentation method and enzyme catalysis method. Enzyme catalysis method has gradually become the mainstream direction because of its advantages of green, environmental protection and pollution-free. And then the purity of NAD powder will reach 99% after the procedure of further purifying. But some NAD manufacturers tend to choose the multiple methods to develop the producing of NAD.
1.Prevention and treatment of viral-induced inflammatory storms
Scientists have found after extensive research that neo-coronavirus has a mechanism similar to SARS virus to activate inflammatory vesicles NLRP3. and the activation of NLRP3 produces more inflammatory factors, generating excessive inflammation and thus triggering a deadly cytokine storm. This problem can be well addressed by NAD+, which inhibits the activity of NF-κB inflammatory pathway and NLRP3 inflammasome by increasing the activity of sirtuins (SIRT1, SIRT2 and SIRT3), thus preventing cytokine storm caused by excessive inflammation. Therefore, Sinclair and other scientists believe that increasing the concentration of NAD+ may play an important role in the prevention and treatment of neocoronavirus and other viral infections.
2.Restoration of virus-induced metabolic disorders
NAD+ is an essential coenzyme for many cellular energy metabolic pathways, present in every cell of the body, involved in thousands of reactions, and an important player in maintaining cellular viability. In the COVID-19 infection model, NAD+ and NMN supplementation was found to be effective in alleviating cell death and protecting the lung.
First, inspect the factory. After some screening, NAD manufactures that directly face consumers pay more attention to brand building. Therefore, for a good brand, quality is the most important thing, and the first thing to control the quality of raw materials is to inspect the factory. Bontac company actually manufacturing NAD powder of high quality with the caterias of SGS. Secondly, the purity is tested. Purity is one of the most important parameters of NAD powder. If high purity NAD cannot be guaranteed, the remaining substances are likely to exceed the relevant standards. As the attached certificates demonstrates that the NAD powder produced by Bontac reach the purity of 99.9%. Finally, a professional test spectrum is needed to prove it. Common methods for determining the structure of an organic compound include Nuclear Magnetic Resonance Spectroscopy (NMR) and high-resolution mass spectrometry (HRMS). Usually through the analysis of these two spectra, the structure of the compound can be preliminarily determined.
In the international market, NAD+ food supplements are in production in the United States, the Australian Food and Drug Administration has approved NAD+ products for sale, and Japanese NAD+ manufactures are devoted to extend the market share in China. Li Ka-shing invested HK$200 million in the US NAD manufacturer, whose NAD+ products are being sold by Watsons in Hong Kong! Then Mclane, a company owned by Warren Buffett also joined the NAD market.
Introduction The crucial parts of nicotinamide adenine dinucleotide (NAD+) and its metabolites in aging and neurodegeneration have been widely recognized. To spur progress toward biochemical research and interventions targeting aging and neurodegenerative diseases, it is of great significance to accurately quantify NAD+ and its metabolite levels in the NAD+ salvage pathway. Here, a robust and accurate LC-MS/MS method is applied to quantify NAD+ and its metabolites levels in normal and injured mouse sciatic nerve. Limitations of existing methods for quantifying NAD+ and its metabolites Traditional methods for quantifying NAD+ and its metabolites, such as HPLC-UV, NMR, capillary zone electrophoresis, or colorimetric enzymatic assays, face various challenges in sensitivity, selectivity, and indirect measurement. As for existing LC-MS/MS assays for cellular or tissue NAD+ and its metabolites measurements, there are still many difficulties to overcome, such as extended run times, poor chromatographic retention behavior, and unsatisfactory peak shapes. Moreover, only one to three substances in the NAD+ salvage pathway can be covered by these methods. The modifications of LC-MS/MS method On the basis of existing LC-MS/MS assays, the modifications regarding the chromatographic conditions, surrogate matrix and MS/MS conditions are conducted. Specifically, 5 μM of methylene phosphonic acid is employed as the mobile phase additive, which explicitly promotes the signal intensity and peak shape. Given the relatively clean and simple nature of never samples and their small size, ultrapure water is tested as a substitute matrix. Instead of hydrophilic interaction liquid chromatography column and hypercarb column, the Waters Atlantis Premier BEH C18 AX column is utilized, whose unique MaxPeak HPS high-performance surface technology (passivating the column inner wall, eliminating metal surface) enables the high reproducibility, peak symmetry, and baseline separation of all analytes. Besides, MS conditions are optimized to minimize the NAD+ interference signal in the cyclic adenosine diphosphate ribose (cADPR) channel while maintaining the response of cADPR and nicotinamide mononucleotide (NMN), with 4000V for ion spray voltage, 450℃ for turbo heater temperature, 50 psi for Gas 1, 50 psi for Gas 2, 30 psi for curtain gas, and 12 psi for collision gas. Representative chromatogram of nerve samples (normal vs injured) All five analytes achieve baseline separation, where cADPR is a sensitive biomarker in the neurodegeneration model. Herein, sciatic nerve axotomy induces axonal degeneration, leading to reduced NAD+ level and elevated NMN level in the injured nerves, resulting in about a 2-fold increase in the NMN/NAD+ ratio. Simultaneously, the levels of nicotinamide (NAM) and adenosine diphosphate ribose (ADPR), are decreased by about 2-fold, while cADPR level is increased by more than 8-fold. These results are consistent with those of previously reported research, verifying the accuracy of this modified LS-MS/MS method in quantifying NAD+ and its metabolites. Conclusion This modified LC-MS/MS method enables effective baseline separation of NAD+, NMN, NAM, ADPR, and cADPR within a brief runtime of 5 min, which is contributive to early diagnoses of various neurological disorders and drug development for aging and neurodegenerative diseases. Reference Ma Y, Deng L, Du Z. Development and validation of an LC-MS/MS method for quantifying NAD+ and related metabolites in mice sciatic nerves and its application to a nerve injury animal model. J Chromatogr A. doi:10.1016/j.chroma.2024.464821 BONTAC NAD BONTAC has been dedicated to the R&D, manufacture and sale of raw materials for coenzyme and natural products since 2012, with self-owned factories, over 170 global patents as well as strong R&D team. BONTAC has rich R&D experience and advanced technology in the biosynthesis of NAD and its precursors (eg. NMN and NR). There are various types of NAD to be selected, encompassing NAD ER Grade (endoxin removal), NAD Grade I (IVD/dietary supplement/cosmetics raw powder), NAD Grade II (API/intermediates) and NAD Grade IV (if any higher requirement on the solubility), which can be provided in the form of lyophilized powder or crystalline powder. The purity of BONTAC NAD can reach above 98%. Disclaimer This article is based on the reference in the academic journal. The relevant information is provided for sharing and learning purposes only, and does not represent any medical advice purposes. If there is any infringement, please contact the author for deletion. The views expressed in this article do not represent the position of BONTAC. Under no circumstances will BONTAC be held responsible or liable in any way for any claims, damages, losses, expenses or costs resulting or arising directly or indirectly from your reliance on the information and material on this website.
Pioneering Discoveries: BONTAC's Innovations in Biotechnical Research In the dynamic realm of biotechnical research, BONTAC stands at the forefront of innovation, dedicated to driving progress and breakthroughs in the field. Our commitment to excellence is demonstrated through our pioneering research initiatives, cutting-edge products, and unwavering focus on technical innovation. At BONTAC, we continuously strive to push the boundaries of biotechnical research to revolutionize industries and improve life. Advancing Biotechnical Research at BONTAC Within BONTAC's research laboratories, a culture of exploration and discovery fuels our quest for innovative solutions in biotechnical research. Our team of experts combines scientific expertise with creative insights to tackle complex challenges and uncover new opportunities. BONTAC's investment in state-of-the-art technology and research facilities underscores our dedication to advancing biotechnical research for the betterment of society. Impactful Applications of Biotechnical Research The fruits of BONTAC's biotechnical research are evident in our array of products that harness the power of scientific insights and technological advancements. These products are the result of meticulous research and development processes aimed at enhancing various sectors such as healthcare, agriculture, and environmental sustainability. From novel treatment options to sustainable agricultural solutions, BONTAC's biotechnical innovations are reshaping industries and driving positive change. At BONTAC, we understand that cellular rejuvenation is not a one-size-fits-all approach. Every individual possesses unique cellular characteristics and experiences distinct environmental factors that influence their overall well-being. That is why we take a personalized approach to cellular rejuvenation, tailoring our solutions to meet the specific needs of each individual. By recognizing the diversity of our customers and their unique cellular profiles, we aim to provide targeted interventions that optimize cellular health and unlock the potential for enhanced vitality. Collaboration and Partnerships in Biotechnical Research Collaboration lies at the heart of BONTAC's approach to biotechnical research, as we recognize the value of partnering with industry leaders and experts to accelerate innovation. By fostering strategic alliances and engaging with key stakeholders, BONTAC creates a network of excellence that fosters collaboration, knowledge exchange, and collective progress in biotechnical research. Altogether, we aim to shape the future of biotechnical innovation and deliver impactful solutions to global challenges. Conclusion In conclusion, BONTAC's dedication to pioneering discoveries in biotechnical research sets us apart as a leader in the field. Our focus on excellent products, robust technical support, and a commitment to technical innovation propels us toward groundbreaking advances that benefit society as a whole. Join us on this exciting journey as we continue to explore new frontiers in biotechnical research, where innovation meets excellence.
Nourishing Wellness: BONTAC as a Leading Raw Material Supplier of Supplements As a prominent raw material supplier of supplements, BONTAC is dedicated to developing and delivering premium products that promote health and well-being through innovative formulations and exceptional quality. We are committed to developing and delivering premium raw materials that fuel the success of your health and wellness products. With our focus on technical innovation, comprehensive business coverage, and dedicated technical support, we stand out as a leading partner for your raw material needs. Expertise in Nutraceutical Formulations BONTAC boasts a team of experts who excel in the development of nutraceutical formulations. Our blend of scientific knowledge and creativity enables us to create effective and advanced formulations that meet the highest standards of quality and efficacy. Through rigorous research and stringent quality control measures, we ensure that our raw materials contribute to the optimal results and success of your supplements. Diverse Range of Nutraceutical Products Deepening our understanding of cellular rejuvenation has been a core focus at BONTAC. We delve into various scientific disciplines, from molecular biology and genetics to epigenetics and regenerative medicine, to unravel the intricate processes that govern cellular aging and rejuvenation. Armed with this knowledge, we develop cutting-edge raw materials that promote cellular rejuvenation, allowing your products to tap into the body's natural ability to restore and regenerate itself. A Diverse Range of Nutraceutical Solutions Exploring BONTAC's extensive range of nutraceutical raw materials reveals a diverse array of products tailored to address various health needs. From immune support and stress management to dietary supplements and performance enhancers, each raw material is carefully crafted to deliver specific benefits. Our innovative ingredients, unique formulations, and proven efficacy in supporting overall health and wellness set our nutraceutical offerings apart from the competition. Partnering with BONTAC for Nutraceutical Excellence By partnering with BONTAC, businesses in the supplements industry can access customized strategies and high-quality raw materials that align with their health and wellness initiatives. Our dedicated technical support team will guide you in selecting the right raw materials and seamlessly integrating them into your product offerings. With BONTAC as your trusted partner, you can leverage our expertise, technical innovations, and extensive business coverage to elevate your product lines and provide exceptional value to your customers. Conclusion In conclusion, BONTAC's position as a leading raw material supplier of nutraceuticals stems from our unwavering commitment to excellence, technical innovation, and exceptional raw materials. By choosing BONTAC as your preferred supplier, you gain access to our expertise, quality raw materials, and dedication to helping your nutraceutical business thrive. Partner with us today and unlock the full potential of your health and wellness products.