Advantages of NMNH
NMNH: 1. “Bonzyme” Whole-enzymatic method, environmental-friendly, no harmful solvent residues manufacturing powder. 2. Bontac is a very first manufacture in the world to produce the NMNH powder on the level of high purity, stability. 3. Exclusive “Bonpure” seven-step purification technology, high purity（up to 99%) and stability of production of NMNH powder 4. Self-owned factories and obtained a number of international certifications to ensure high quality and stable supply of products of NMNH powder 5. Provide one-stop product solution customization service
Advantages of NADH
NADH: 1. Bonzyme whole-enzymatic method, environmental-friendly, no harmful solvent residues 2. Exclusive Bonpure seven-step purification technology, purity up higher than 98 % 3. Special patented process crystal form, higher stability 4. Obtained a number of international certifications to ensure high quality 5. 8 domestic and foreign NADH patents, leading the industry 6. Provide one-stop product solution customization service
Advantages of NAD
NAD: 1. “Bonzyme” Whole-enzymatic method, environmental-friendly, no harmful solvent residues 2. Stable supplier of 1000+ enterprises around the world 3. Unique “Bonpure” seven-step purification technology, higher product content and higher conversion rate 4. Freeze drying technology to ensure stable product quality 5. Unique crystal technology, higher product solubility 6. Self-owned factories and obtained a number of international certifications to ensure high quality and stable supply of products
Advantages of MNM
NMN: 1. “Bonzyme”Whole-enzymatic method, environmental-friendly, no harmful solvent residues 2. Exclusive“Bonpure”seven-step purification technology, high purity（up to 99.9%) and stability 3. Industrial leading technology: 15 domestic and international NMN patents 4. Self-owned factories and obtained a number of international certifications to ensure high quality and stable supply of products 5. Multiple in vivo studies show that Bontac NMN is safe and effective 6. Provide one-stop product solution customization service 7. NMN raw material supplier of famous David Sinclair team of Harvard University
We Have The Best Solutions for Your Business
Bontac Bio-Engineering (Shenzhen) Co., Ltd. (hereafter referred to as BONTAC) is a high-tech enterprise established in July 2012. BONTAC integrates R&D, production and sales, with enzyme catalysis technology as the core and coenzyme and natural products as main products. There are six major series of products in BONTAC, involving coenzymes, natural products, sugar substitutes, cosmetics, dietary supplements and medical intermediates.
As the leader of the global NMN industry, BONTAC has the first whole-enzyme catalysis technology in China. Our coenzyme products are widely used in health industry, medical & beauty, green agriculture, biomedicine and other fields. BONTAC adheres to independent innovation, with more than 170 invention patents. Different from the traditional chemical synthesis and fermentation industry, BONTAC has advantages of green low-carbon and high-value-added biosynthesis technology. What’s more, BONTAC has established the first coenzyme engineering technology research center at the provincial level in China which also is the sole in Guangdong Province.
In the future, BONTAC will focus on its advantages of green, low-carbon and high-value-added biosynthesis technology, and build ecological relationship with academia as well as upstream/downstream partners, continuously leading the synthetic biological industry and creating a better life for human beings.
NAD powder manufacturing method
The preparation methods of NAD powder are mainly divided into chemical synthesis method and biocatalytic method, among which biocatalytic method includes biological fermentation method and enzyme catalysis method. Enzyme catalysis method has gradually become the mainstream direction because of its advantages of green, environmental protection and pollution-free. And then the purity of NAD powder will reach 99% after the procedure of further purifying.
NAD powder efficacy in health
Molecules that can be taken in supplement form to increase NAD levels in the body are referred to by some as “NAD boosters.” Studies conducted over the past six decades suggest that the following are some of the many benefits associated with taking an NAD supplement:
Can Help Restore Mitochondrial Function
Helps Repair Blood Vessels —A 2018 mice study found that supplementation could aid in repair and growth of aged blood vessels. There’s also some evidence it can help manage heart disease risk factors like high blood pressure and high cholesterol.
May Improve Muscle Function — One animal study conducted in 2016 found that degenerative muscles had improved muscle function when supplemented with NAD+ precursors.
Potentially Helps Repair Cells and Damaged DNA — Some studies have found evidence that NAD+ precursor supplementation leads to an increase in DNA damage repair. NAD+ is broken down into two component parts, nicotinamide and ADP-ribose, which combine with proteins to repair cells.
May Help Improve Cognitive Function — Several studies conducted on mice have found that mice treated with NAD+ precursors experienced improvements in cognitive function, learning and memory. Findings have led researchers to believe that NAD supplement may help protect against cognitive decline/Alzheimer’s disease.
May Help Prevent Age-Related Weight Gain — A 2012 study showed that when mice fed a high-fat diet were given an NAD supplement, they gained 60 percent less weight than they did on the same diets without the supplement. One reason this may be true is that nicotinamide adenine dinucleotide helps regulate production of stress- and appetite-related hormones, thanks to its effects on circadian rhythms.
Precursors are molecules used in chemical reactions inside the body to create other compounds. There are a number of precursors of NAD+ that result in higher levels when you consume enough of them.
BONTAC NAD product features and advantages
1、Enzymatic method, environmental-friendly, no harmful solvent residues manufacturing powder
2、High purity（up to 99%) and stability of production of NAD powder
3、Self-owned factories and obtained a number of international certifications to ensure high quality and stable supply of products of NAD powder
4、Multiple in vivo studies show that Bontac NAD powder is safe and effective
5、Provide one-stop product solution customization service
User Reviews
What users say about BONTAC
Frequently Asked Question
Do you have any question?
Nicotinamide adenine dinucleotide (NAD) has several essential roles in metabolism. It acts as a coenzyme in redox reactions, as a donor of ADP-ribose moieties in ADP-ribosylation reactions, as a precursor of the second messenger molecule cyclic ADP-ribose, as well as acting as a substrate for bacterial DNA ligases and a group of enzymes called sirtuins that use NAD+ to remove acetyl groups from proteins. In addition to these metabolic functions, NAD+ emerges as an adenine nucleotide that can be released from cells spontaneously and by regulated mechanisms, and can therefore have important extracellular roles.
First, inspect the factory. After some screening, NAD companied that directly face consumers pay more attention to brand building. Therefore, for a good brand, quality is the most important thing, and the first thing to control the quality of raw materials is to inspect the factory. Bontac company actually manufacturing NAD powder of high quality with the caterias of SGS. Secondly, the purity is tested. Purity is one of the most important parameters of NAD powder. If high purity NAD cannot be guaranteed, the remaining substances are likely to exceed the relevant standards. As the attached certificates demonstrates that the NAD powder produced by Bontac reach the purity of 99.9%. Finally, a professional test spectrum is needed to prove it. Common methods for determining the structure of an organic compound include Nuclear Magnetic Resonance Spectroscopy (NMR) and high-resolution mass spectrometry (HRMS). Usually through the analysis of these two spectra, the structure of the compound can be preliminarily determined.
The difference all comes down to the charge of these coenzymes. NAD+ is written with a superscript + sign because of the positive charge on one of its nitrogen atoms. It is the oxidized form of NAD. It’s considered “an oxidizing agent” because it accepts electrons from other molecules.
Although they are different chemically, these terms are mostly used interchangeably when discussing their health benefits. Another term you may come across is NADH, which stands for nicotinamide adenine dinucleotide (NAD) + hydrogen (H). This is also used interchangeably with NAD+ for the most part. Both are nicotinamide adenine dinucleotides that function as either hydride donors or hydride acceptors. The difference between these two is that that NADH becomes NAD+ after it donates an electron to another molecule.
Our updates and blog posts
Targeting NAD+ Salvage Pathway as a Potential Approach to Combat Obesity
Introduction Mar 4th is determined as the World Obesity Day. World Obesity Federation, UNICEF and WHO have hosted a global youth-led webinar to talk about obesity & youth. The obesity crisis has gradually attracted much attention. The latest report by the Lancet suggests that one billion people are bothered by obesity (2022), with 650 million adults, 340 million adolescents and 39 million children. Recently, etiological studies and interventions for obesity have been progressively focused on the central nervous system, with an attempt to curb the onset of obesity at its source. Notably, targeting NAD+ salvage pathway in hypothalamic astrocytes may be a potential approach to combat obesity. The association of hypothalamic astrocytes and obesity The hypothalamus functions as the appetite regulation center, which receives and integrates the neuroendocrine factors produced by the central nervous system and peripheral tissues to promote or suppress appetite, so as to affect body weight. Noteworthily, aypothalamic astrocytes can apparently decrease glucose clearance and increase plasma insulin levels, playing an essential role in modulating energy metabolism, which are expected to be a new target for obesity treatment. Alleviation of high-fat diet (HFD)-induced obesity by repressing astrocyte NAD+ salvage pathway Under conditions of excessive fat intake, the NAD+ salvage pathway is specifically activated in hypothalamic astrocytes, which restrains the energy expenditure (EE) and fat oxidation in adipose tissues by downregulating sympathetic nerve innervation, eventually resulting in the accumulation of adipose tissue fat and the development of obesity. CD38 as a downstream mediator of astrocyte inflammation induced by the NAD+ salvage pathway. CD38 functions downstream of the NAD+ salvage pathway in hypothalamic astrocytes burdened with excess fat. CD38 knockdown in arcuate nucleus astrocytes diminishes the weight gain, reduces fat mass, increases EE, and lowers RER during HFD consumption. Cd38 depletion in hypothalamic astrocytes may improve hypothalamic inflammation by increasing NAD+ level. Hypothalamic inflammation can not only lead to energy imbalances, but also exacerbate central insulin resistance and leptin resistance, which can lead to the accumulation of fat in peripheral tissues. The role of nicotinamide phosphoribosyltransferase (NAMPT)–NAD+–CD38 axis in obesity In mammals, the salvage pathway represents the primary means of maintaining cellular NAD+ level. A crucial step in the NAD+ salvage pathway is catalyzed by NAMPT. In response to fat overload, the activation of the astrocytic NAMPT-NAD+-CD38 axis induces pro-inflammatory responses in the hypothalamus, eliciting aberrantly activated basal Ca2+ signals and compromised Ca2+ responses to metabolic hormones such as insulin, leptin, and glucagon-like peptide 1, ultimately resulting in dysfunctional hypothalamic astrocytes and contribute to the development of obesity. Conclusion Mechanically, inhibition of hypothalamic astrocytic NAD+ salvage pathway, along with its downstream CD38, mitigates hypothalamic inflammation and attenuates the development of HFD-induced obesity in male mice. Reference Park, J.W., Park, S.E., Koh, W. et al (2024). Hypothalamic astrocyte NAD+ salvage pathway mediates the coupling of dietary fat overconsumption in a mouse model of obesity. Nat Commun 15, 2102. https://doi.org/10.1038/s41467-024-46009-0 BONTAC NAD BONTAC has been dedicated to the R&D, manufacture and sale of raw materials for coenzyme and natural products since 2012, with self-owned factories, over 170 global patents as well as strong R&D team. BONTAC has rich R&D experience and advanced technology in the biosynthesis of NAD and its precursors (eg. NMN and NR). There are various types of NAD to be selected, encompassing NAD ER Grade (endoxin removal), NAD Grade I (IVD/dietary supplement/cosmetics raw powder), NAD Grade II (API/intermediates) and NAD Grade IV (if any higher requirement on the solubility), which can be provided in the form of lyophilized powder or crystalline powder. The purity of BONTAC NAD can reach above 98%. Disclaimer This article is based on the reference in the academic journal. The relevant information is provided for sharing and learning purposes only, and does not represent any medical advice purposes. If there is any infringement, please contact the author for deletion. The views expressed in this article do not represent the position of BONTAC. BONTAC holds no responsibility for any claims, damages, losses, expenses or costs resulting or arising directly or indirectly from your reliance on the information and material on this website.
Extraordinary Merits of Ultrasensitive ELISA with Thio-NAD Cycling in Determining Dengue NS1 Protein
1. Introduction Dengue fever is an acute infectious disease caused by the Dengue virus through the bite of an infected Aedes species (Ae. aegypti or Ae. albopictus) mosquito, with the main symptoms such as high fever, dizziness, headache, rash and severe pains (eye, muscle, joint, or bone pain), etc. This disease is widely prevalent in tropical and subtropical climates. An estimated 100 to 400 million people are infected each year, where over 80% are usually mild and asymptomatic. Nonetheless, severe Dengue fever can increase the risk of death if not treated properly. Thus, early diagnosis of this disease is significant to its timely treatment. Noteworthily, non-structural protein 1 (NS1), a highly conserved glycoprotein, is chiefly secreted during the infection of Dengue virus, which is intensively deemed as the main pathogenesis factor of Dengue fever. Hence, NSI is generally used as a biomarker for the early detection of this disease. In this study, a sandwich enzyme-linked immunosorbent assay (ELISA) in combination with the thio nicotinamide adenine dinucleotide (thio-NAD/S-NAD) cycling method (hereafter termed ultrasensitive ELISA) is utilized to detect NSI, followed by the comparison with NAAT to confirm its detection accuracy. 2. Advantages and disadvantages of traditional detection methods for Dengue fever At present, there are four main detection methods for Dengue fever. Viral isolation and identification have high specificity but are time-consuming, taking at least 5 days. The rapid antigen test is the fastest and most cost-efficient among the other methods, but the sensitivity and specificity are relatively low. Serologic test based on IgM and IgG is limited by the number of days of infection, as the test must be delayed until the level of antibodies rises to a detectable level. In clinic, NAAT is often applied to determine Dengue fever by dirt of its high sensitivity and specificity. However, this method is expensive, laborious, and prone to false positivity, which must be conducted by the trained personnel. To overcome the disadvantages of these methods, a new detection method ultrasensitive ELISA is applied in this study. 3. Workflow of ultrasensitive ELISA with thio-NAD cycling A pair of antibodies is used for capturing the NS1 protein in the sandwich ELISA, and alkaline phosphatase is labeled on the secondary antibody. Aside from the antibodies, an androsterone derivative, 3α-hydroxysteroid dehydrogenase, thio-NAD, and NADH are used to construct the thio-NAD enzyme cycling system. During the thio-NAD cycling reaction, thio-NADH constantly accumulated in a triangular number fashion and could be directly measured at an absorbance of 405 nm. 4. The comparison of ultrasensitive ELISA and NAAT in the detection of Dengue NS1 Protein In NAAT, 60 specimens are dengue-positive, and 25 are dengue-negative. The NAAT cycling threshold (CT) value of those dengue-positive specimens ranged from 12.42 to 31.41. In the ultrasensitive ELISA, 59 specimens are correspondingly positive to the NAAT results, whereas 25 specimens are completely correspondingly negative to the NAAT results. Compared with NAAT, the sensitivity and specificity of the ultrasensitive ELISA are 98.3% and 100%, respectively (Table 2). Of 60 NAAT-confirmed dengue-positive patient specimens, only 1 specimen is negative in the ultrasensitive ELISA. The NAAT data showed that the specimen is a type 4 DENV infection case with a CT value of 21.59. The results of the ultrasensitive ELISA are in almost perfect agreement with the NAAT results, with a kappa value of 0.972 (95% CI: 0.917-1.0). 5. Conclusion Ultrasensitive ELISA method is easy to perform and requires no professional trainees to operate. The detection can be started immediately after receiving a small sample. It is particularly suitable for the early detection of Dengue disease in low-income countries. Reference Chen, Po-Kai et al. “Advanced Detection Method for Dengue NS1 Protein Using Ultrasensitive ELISA with Thio-NAD Cycling.” Viruses vol. 15,9 1894. 8 Sep. 2023, doi:10.3390/v15091894 Product advantages and features of BONTAC Thio-NAD/S-NAD BONTAC is one of the few enterprises in China that can produce Thio-NAD, with “Bonzyme” Whole-enzymatic method (environmental-friendly; no harmful solvent residues) and unique “Bonpure” seven-step purification technology. BONTAC intergrates R&D, production and sale, with self-owned factories and a number of international certifications to ensure high quality and stable supply of products. Disclaimer BONTAC shall hold no responsibility for any claims arising directly or indirectly from your reliance on the information and material on this website.
Rg3-PLGA@TMVs May Be the Candidates for Potential Inclusion in Cancer Therapy
Introduction Natural products, including single components or extracts of herbal medicines, have the features of good efficacy and low toxicity when used in tumor treatment. They have unique advantages in treating diseases compared with chemotherapeutic drugs due to their multi-component and multi-target effects. Strikingly, ginsenoside Rg3-loaded PLGA nanoparticles (Rg3-PLGA) coated with the membrane of tumor cell-derived microvesicles (Rg3-PLGA@TMVs), which is a nanoformulation of the natural product ginsenoside Rg3, have a significant role in the treatment of breast cancer when combined with the chemotherapeutic drug doxorubicin (DOX). Pharmacological roles of ginseng Rg3 in traditional Chinese medicine Ginseng Rg3, a tetracyclic triterpenoid saponin, is one of the main active component of ginseng. Ginseng has been regarded as one of the most valuable medicinal plants in Chinese traditional medicine for thousands of years, which is known as "the king of all tonic”. This perennial herb possesses the multiple pharmaceutical functions such as tonifying the vital energy, restoring the pulse and stabilizing the loss of vitality, invigorating spleen and benefiting lung, generating saliva or body fluid to quench thirst, as well as tranquilizing the mind and promoting the intelligence. The construction of Rg3-PLGA@TMVs Ginsenoside Rg3 is first encapsulated into PLGA nanoparticles using the nanoprecipitation method, followed by the extrusion-based coating of the TMV membrane onto Rg3-PLGA. This strategy combines the therapeutic properties of Rg3 with the precise delivery and sustained release capabilities of PLGA nanoparticles, further enhanced by the homologous targeting properties of TMVs. Anti-tumor effect and bio-safety of Rg3-PLGA@TMVs in 4T1 breast cancer Rg3-PLGA@TMVs induce robust anti-tumor immunity, recover the body weight loss caused by DOX in 4T1 breast cancer and overtly improve the anti-tumor effect of DOX, showing good stability and drug loading capability. These anti-tumor effects are chiefly realized by reducing phagocytic activity and acid phosphatase (ACP) levels, promoting the maturation of bone marrow-derived dendritic cells in vitro, and elevating the population of cytotoxic T lymphocytes (CD3+CD8+) and helper T cells (CD3+CD4+) in vivo. Importantly, this nanoformulation has minimal side effects and favorable biocompatibility, which can relieve the organ toxicity caused by DOX, especially the cardiac side effects. The administration of DOX and Rg3-PLGA@TMVs does not result in statistically significant changes in white blood cell (WBC) count and renal functional indicators, such as CREA and urea, nor in hepatic functional indicators, including AST and ALT. Conclusion Rg3-PLGA@TMVs achieve the co-delivery of antigens and adjuvants, which not only potentiate the anti-tumor effects, but also significantly alleviate the systemic toxicity caused by chemotherapeutic drugs, improving the overall health status of patients undergoing chemotherapy. Reference [1] Liu XR, Zhang KF, Li X, et al. Research progress on antitumor nanoscale drug delivery system of ginsenoside Rg3 [J]. Chin Trad Herb Drugs. 2023,54(22):7577-7587. DOI: 10.7501/j.issn.0253-2670.2023.22.034 [2]Zhang S, Zheng B, Wei Y, et al. Bioinspired ginsenoside Rg3 PLGA nanoparticles coated with tumor-derived microvesicles to improve chemotherapy efficacy and alleviate toxicity [J]. Biomater Sci. Published online April 10, 2024. DOI:10.1039/d4bm00159a BONTAC Ginsenosides BONTAC has been dedicated to the R&D, manufacture and sale of raw materials for coenzyme and natural products since 2012, with self-owned factories, over 170 global patents as well as strong R&D team. BONTAC has rich R&D experience and advanced technology in the biosynthesis of rare ginsenosides Rh2/Rg3, with pure raw materials, higher conversion rate and higher content (up to 99%). With unique Bonzyme enzymatic synthesis technology, both S-type and R-type isomers can be accurately synthesized here, with stronger activity and precise targeting action. Our products are subjected to strict third-party self-inspection, which are worth of trustworthy. Disclaimer This article is based on the reference in the academic journal. The relevant information is provided for sharing and learning purposes only, and does not represent any medical advice purposes. If there is any infringement, please contact the author for deletion. The views expressed in this article do not represent the position of BONTAC. Under no circumstances will BONTAC be responsible for any claims, damages, losses, expenses, or costs arising directly or indirectly from your reliance on the information and material on this website.